Wednesday, January 31, 2024

The Terrifying & Frankensteinian Project to Bio-Engineer Your Proteins


Transfected mRNA in endothelial brain cells is Frankensteinian and raises very real risks for protein folding disorders given what we now know about "frameshifting" of protein production induced by the mRNA vaccines. (Note these are actually synthetic mRNA see my post here ).

Let us start by looking at the research article claiming to penetrate the blood-brain barrier using mRNA encased in lipid nanoparticles (LNPs):

Emily L. Han, Marshall S. Padilla, Rohan Palanki, Dongyoon Kim, Kaitlin Mrksich, Jacqueline J. Li, Sophia Tang, Il-Chul Yoon, and Michael J. Mitchell (2024). Predictive High-Throughput Platform for Dual Screening of mRNA Lipid Nanoparticle Blood–Brain Barrier Transfection and Crossing. Nano Lett. 2024, https://doi.org/10.1021/acs.nanolett.3c03509

Lipid nanoparticle (LNP)-mediated nucleic acid therapies, including mRNA protein replacement and gene editing therapies, hold great potential in treating neurological disorders including neurodegeneration, brain cancer, and stroke.
However, delivering LNPs across the blood–brain barrier (BBB) after systemic administration remains underexplored. In this work, we engineered a high-throughput screening transwell platform for the BBB (HTS-BBB), specifically optimized for screening mRNA LNPs.
Unlike most transwell assays, which only assess transport across an endothelial monolayer, HTS-BBB simultaneously measures LNP transport and mRNA transfection of the endothelial cells themselves.
We then use HTS-BBB to screen a library of 14 LNPs made with structurally diverse ionizable lipids and demonstrate it is predictive of in vivo performance by validating lead candidates for mRNA delivery to the mouse brain after intravenous injection. Going forward, this platform could be used to screen large libraries of brain-targeted LNPs for a range of protein replacement and gene editing applications.

  • Read Press Release here: https://penntoday.upenn.edu/news/bioengineers-brink-breaching-blood-brain-barrier
  • Also see publicity here:Tim Newcomb (2024, Jan 31). Scientists are about to break the blood-brain barrier- and maybe transform medicine. Popular Mechanics. https://www.popularmechanics.com/science/health/a46584155/blood-brain-barrier/

SO, what is being developed is a technique for bio-engineering how your brain cells produce proteins. NOW LET'S LOOK AT WHAT WE KNOW ABOUT FRAME-SHIFTING and explore the implications for how your body/brain produces proteins:

First, here is a press-release exploring frameshifting, or the glitching of protein production induced by the mRNA vaccines:

Pinkstone, Joe. (2023, December). Science Correspondent 6 December 2023 One in four who had Pfizer Covid jabs experienced unintended immune response https://www.telegraph.co.uk/news/2023/12/06/mrna-jabs-modena-pfizer-quarter-unintended-response/

mRNA vaccines were affected by the glitch but no adverse effects were created, Cambridge researchers say More than a quarter of people injected with mRNA Covid jabs suffered an unintended immune response created by a glitch in the way the vaccine was read by the body, a study has found. No adverse effects were created by the error, data show, but Cambridge scientists found such vaccines were not perfect and sometimes led to nonsense proteins being made instead of the desired Covid “spike”, which mimics infection and leads to antibody production. mRNA jabs, such as the ones created by Moderna and Pfizer, use a string of genetic material to tell the body to create a specific protein that safely imitates an infection. Research in the field, spanning decades, had been slow work. It often stalled because RNA itself is often attacked by the body as a foreign invader.

HERE IS THE RESEARCH STUDY upon which the press release is based:

Mulroney, T.E., Pöyry, T., Yam-Puc, J.C. et al. N1-methylpseudouridylation of mRNA causes +1 ribosomal frameshifting. Nature (2023). https://doi.org/10.1038/s41586-023-06800-

In vitro-transcribed (IVT) mRNAs are modalities that can combat human disease, exemplified by their use as vaccines for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). IVT mRNAs are transfected into target cells, where they are translated into recombinant protein, and the biological activity or immunogenicity of the encoded protein exerts an intended therapeutic effect1,2.

Modified ribonucleotides are commonly incorporated into therapeutic IVT mRNAs to decrease their innate immunogenicity3,4,5, but their effects on mRNA translation fidelity have not been fully explored. Here we demonstrate that incorporation of N1-methylpseudouridine into mRNA results in +1 ribosomal frameshifting in vitro and that cellular immunity in mice and humans to +1 frameshifted products from BNT162b2 vaccine mRNA translation occurs after vaccination.

The +1 ribosome frameshifting observed is probably a consequence of N1-methylpseudouridine-induced ribosome stalling during IVT mRNA translation, with frameshifting occurring at ribosome slippery sequences. However, we demonstrate that synonymous targeting of such slippery sequences provides an effective strategy to reduce the production of frameshifted products.

Overall, these data increase our understanding of how modified ribonucleotides affect the fidelity of mRNA translation, and although there are no adverse outcomes reported from mistranslation of mRNA-based SARS-CoV-2 vaccines in humans, these data highlight potential off-target effects for future mRNA-based therapeutics and demonstrate the requirement for sequence optimization.
BASICALLY, the process for engineering proteins is FULL OF ERRORS as the directions are subjected to frameshifting errors. So, mRNA produces MIS-FOLDED PROTEINS. 

I do not want misfolded proteins anywhere in my body, especially IN MY BRAIN.

IN OTHER NEWS - LEARN HOW SARS-CoV-2 viral proteins self-assemble into RNA that contribute to catastrophic auto-immune attacks:

SARS-CoV-2 viral fragments self-assemble into "new structures with double-stranded RNA, a special molecule essential for building proteins from DNA" and the resulting "hybrid complex of immune peptides and double-stranded RNA" produces a strong immune response.

California NanoSystems Institute JANUARY 29, 2024Viral protein fragments may unlock mystery behind serious COVID-19 outcomes https://medicalxpress.com/news/2024-01-viral-protein-fragments-mystery-covid.html

In a study appearing in the journal Proceedings of the National Academy of Sciences, a UCLA-led multidisciplinary research team explores one way that COVID-19 turns the immune system—which is crucial for keeping people alive—against the body itself, with potentially deadly results.

Using an artificial intelligence system they developed, the study authors scanned the entire collection of proteins produced by SARS-CoV-2 and then performed an exhaustive series of validation experiments. 

The scientists found that certain viral protein fragments, generated after the SARS-CoV-2 virus is broken down into pieces, can mimic a key component of the body's machinery for amplifying immune signals.

Their discoveries suggest that some of the most serious COVID-19 outcomes can result from these fragments overstimulating the immune system, thereby causing rampant inflammation in widely different contexts such as cytokine storms and lethal blood coagulation. 

…The research team found SARS-CoV-2 fragments can imitate innate immune peptides, a class of immune molecules that amplify signals to activate the body's natural defenses. Peptides are chains of amino acids like proteins, only shorter. 

These immune peptides can spontaneously assemble into new structures with double-stranded RNA, a special form of a molecule essential for building proteins from DNA, typically found in viral infections or released by dying cells.

The resultant hybrid complex of the immune peptides and double-stranded RNA kicks off a chain reaction that triggers an immune response. 

•More information: Yue Zhang et al, Viral afterlife: SARS-CoV-2 as a reservoir of immunomimetic peptides that reassemble into proinflammatory supramolecular complexes, Proceedings of the National Academy of Sciences (2024). DOI: 10.1073/pnas.2300644120. doi.org/10.1073/pnas.2300644120

 The spike proteins produced by the mRNA vaccines will produce same effects!

RELATED POSTS

http://majiasblog.blogspot.com/2023/09/hazards-of-mrna-vaccine-platform.html


http://majiasblog.blogspot.com/2022/05/mrna-vaccines-linked-to-higher-cardio.html

http://majiasblog.blogspot.com/2022/04/fda-shuts-out-its-own-experts-in.html

http://majiasblog.blogspot.com/2022/03/thanatopolitics-and-vaccine-apartheid.html

http://majiasblog.blogspot.com/2021/08/what-is-going-on-no-public-health.html

http://majiasblog.blogspot.com/2021/06/spike-protein-blues.html

http://majiasblog.blogspot.com/2021/06/approval-mandatory-vaccine-mandates-for.html 

 

http://majiasblog.blogspot.com/2022/05/mrna-vaccines-linked-to-higher-cardio.html

http://majiasblog.blogspot.com/2022/04/fda-shuts-out-its-own-experts-in.html

http://majiasblog.blogspot.com/2022/03/thanatopolitics-and-vaccine-apartheid.html

http://majiasblog.blogspot.com/2021/08/what-is-going-on-no-public-health.html

http://majiasblog.blogspot.com/2021/06/spike-protein-blues.html

http://majiasblog.blogspot.com/2021/06/approval-mandatory-vaccine-mandates-for.html 









Saturday, January 20, 2024

Pluralism and Democracy Imperilled: WEF Coding Pluralism and Dissent as Dis/Mis-information

Apparently our WEF delegate, John Kerry told his peers that US consumer reluctance to fully embrace EVs has nothing to do with their current technological shortcomings and has everything to do with "disinformation" (see here).

The World Economic Forum's Global Risk Report lists "misinformation" and "disinformation" as the leading global threats. This prioritization essentially tells you everything you need to know about that organization and its proponents.

Above all else, they fear opinions, values, and facts that diverge from their narrative. Messy uncertainties and disagreements about interpretations, values and policies must all give way to our elite's elevated Truth, operationalized in their policies and programs.

George Orwell could not have been more prescient in his account of newspeak greasing authoritarianism.

I argue that any group that plans to use discipline (e.g., algorithmic censorship) and force (financial extortion and platform cancellation) to promote their "truth" and cancel alternative sense-making is fundamentally anti-democratic and anti-humanist.

Our elites have slipped into a post-humanist technocratic fantasy that elevates their expertise in remaking humanity according to their dystopian aspirations. Freedom of speech is the greatest obstacle to their tyranny.

Tragically for us peons, the elite ideology of newspeak has circulated so extensively in foundational institutions, fueled by extrinsic (e.g., funding) and intrinsic rewards (e.g., ego), that resistance will not be forthcoming from these quarters.

The contradiction between lived experience, with EVs and experimental injections, and official rhetoric in the "lifeworld" (as described by Jurgen Habermas) will ground resistance. Organic intellectuals will emerge from this space, but the disciplinary panopticism being unveiled by our self-important elites will be difficult to combat.

Violent resistance will unleash their most repressive forces so that option should not be prioritized. Non-violent resistance promoted by figures such as MLK and M. Gandhi. 



Sunday, January 14, 2024

The Perfect Bio-thanatopolitical Crime

 

The idea of the perfect crime is grounded in the narrative of evil's triumph over humanity.

In this narrative, a perfect crime is defined in relation to the collapse of justice and goodness as evil succeeds in its aims and avoids detection.

In this brief essay, I use Foucault's theoretical work on power to illustrate one imagination of the "perfect crime."

...

The Dual Nature of Bio/Thanatopolitical power

In Foucault's work on power, he describes the dual applications of pastoral power, the power rooted in the metaphor of cultivation, that seeks to govern the lifeforces of the population to achieve particular goals, such as security.

The representation and governance of those life forces is biopolitical. Nikolas Rose described biopolitics as the "politics of life itself." (see journal article cited below)

Foucault observed that pastoral biopolitics are usually "productive," in the sense that they are organized around efforts to cultivate life. 

Conversely, deliberate tillings (e.g., eugenics sterilizations; ethnic cleansing), or simply callous, administration of human life forces are not properly pastoral so these forms of administration are called thanatopolitics because they are concerned with killing and letting die, a more passive approach.

R. Esposito, an Italian scholar, has explored how biopolitics inverts as thanatopolitics with amplification of an 'immunological" turning against the "body," of the population body or targeted elements. 

Pastoral biopolitics tips easily into Thanatopolitics. (See also Mbembé& Meintjes; Murray below)

Bio/Thanatopolitical Power and the Perfect Crime

The perfect crime, from a moral perspective that transcends Foucault's materialism, involves the deliberate exploitation of the dual nature of bio/thanatopolitics with destruction in mind.

To avoid detection, the perfect crime is represented as something other than its material effects on targeted populations.

Historically, crimes against human lifeforces have adapted the guises of pastoral benevolence and expertise, as illustrated in the programs of medical police that have been deployed historically ranging from the German medical police through eugenic and experimental denigration and destruction of human life forces, as occurs with medical experimentation (Goliszek, 2003)

In addition to adopting a falsely beneficent pastoral guise, Scapegoating and Gaslighting have historically been used to displace attention from destructive or disciplining forces.

Gaslighting occurs as the Foucauldian "rules of truth" marginalize and stigmatize forbidden or simply subordinated knowledge and experience. The lived experiences of falsehoods and injuries are suppressed entirely or categorically disqualified.

Scapegoating is no doubt one of the oldest tricks for humanity. Other intelligent animals engage in it also. Scapegoating natural hazards affords a sense of control over energies that escape our human disciplines. Scapegoating human failings, particularly thanatopolitical programs designed to exploit and/or subjugate is particularly insidious.

Scapegoating enemies, particularly those who challenge thanatopolitical power, delivers the second requirement of the perfect crime, evasion of accountability for harm.

EXPERIENCE

When I see examples of these perfect crimes I am sickened and saddened. I more often see scapegoating of destruction. Rarely have I ever seen a case until today where those challenging thanatopolitical power are blamed directly for the crimes of the true perpetrators.


SOURCES

Esposito, R. (2008). Bios: Biopolitics and philosophy (T. Campbell, Trans.). Minneapolis: University of Minnesota Press.

Foucault, Michel (20140. The politics of health in the eighteenth century. Foucault Studies, No. 18, pp. 113127.

Foucault, M. (1979). Discipline and punish (A. Sheridan, Trans.). New York: Vintage Books.

Foucault, M. (1983). The subject and power. In H. L. Dreyfus & P. Rabinow (Eds.), Michel Foucault: Beyond structuralism and hermeneutics (pp. 208-264). Chicago: University of Chicago.

Foucault, M. (1990). The history of sexuality: An introduction (R. Hurley Trans.). New York: Vintage.

Foucault, M. (1994). The order of things: An archeology of the human sciences. New York: Vintage.

Foucault, M. (2007). Security, territory, population (M. Senellart, Ed., G. Burchell, Trans.). Houndsmills: Palgrave.

Foucault, Michel. The Birth of Biopolitics: Lectures at the Collège de France 1978-1979. Trans. G. Burchell. Ed. Michel Senellart. Houndmills: Palgrave, 2008.
 

Goliszek, A. (2003) In the Name of Science: A History of Secret Programs, Medical Research, and Human Experimentation. New York: St. Martin’s Press, 125.

Mbembé, J. Achilleand Meintjes, Libby "Necropolitics," Public Culture 15.1 (2003): 11-40. Project MUSE. Web. 29 Aug. 2011. <http://muse.jhu.edu/>,”14.

Murray, Stuart Thanatopolitics http://stuartjmurray.com/thanatopolitics-2/

Nadesan, Majia (2008). Biopower, governmentality and everyday life. New York: Routledge.

Rose, N. (2001). The politics of life itself. Theory, Culture and Society, 18(6), 1-30.


ADDITIONAL READINGS

Creager A (2013) Life Atomic: A History of Radioisotopes in Science and Medicine. Chicago, IL: University of Chicago Press.

P. Langley (2012) Medicine and the Bomb: Deceptions from Trinity to Maralinga (Aldinga Beach, South Australia: Paul Langley), http://pothi.com/pothi/book/ebook-paul-langley-medicine-and-bomb, date accessed 21 December 2012.

Steven Pokornowski Insecure Lives: Zombies, Global Health, and the Totalitarianism of Generalization. Literature and Medicine Volume 31, Number 2, Fall 2013 pp. 216-234 10.1353/lm.2013.0017
 

ABSTRACT•This article examines how biological and political insecurity have become increasingly imbricated with one another. Building on Priscilla Wald's assertion that contagion reveals the connections of an imagined community, I show how it also reveals disconnections: who is dispossessed by these imagined communities. Through examinations of the Resident Evil franchise, Steven Soderbergh's Contagion, and Max Brooks's World War Z, I demonstrate how through contagion, individuals are marked as biologically and politically threatening, dispossessed of an imagined community, and then subjected to violence. In all of these texts, the globalization of an imbricated health and security complex enacts a generalizing logic that obscures local history and cultural specificity. This logic works to dispossess individuals of imagined communities along racialized, gendered, and classed lines, and makes that dispossession appear apolitical. This article advocates instead for a recognition of the history and relation of the terms and figures that populate the cultural imaginary.